UNIVERSITAS AIRLANGGA



Detail Article

Indonesian Journal of Clinical Pathology and Medical Laboratory

ISSN 0854-4263

Vol. 18 / No. 1 / Published : 2011-01

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Original Article :

(comparison of determination for thyroxine with enzyme linked immunofluorescent assay (elfa) and enzyme linked immunosorbant assay (elisa)}

Author :

  1. Faizah Yunianti*1
  2. Siswanto Darmadi*2
  3. M Y. Probohoesodo*3
  4. Budiono*4
  1. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya
  2. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya
  3. Bag PK FK-UNAIR/RSU Dr. Soetomo Surabaya
  4. 2. Ilmu Kesehatan Masyarakat, Fakultas Kedokteran Universitas Airlangga, Surabaya

Abstract :

  The determination of thyroxin (T4) is known as a good indicator to know the condition of thyroid function. In the hyperthyroid state, are shown clearly the increased levels of T4, while in the hypothyroid state their levels always decrease. The T4 levels change due to the physiological and pathological conditions on the ability of thyroxin binding globulin (TBG). The T4 measurement can be performed using an enzyme-linked fluorescent immunoassay (ELFA) or enzyme-linked immunosorbant assay (ELISA). Both ELFA and ELISA can detect T4 antigen. In these research fifty one randomized samples sera from the Clinical Pathology laboratory, at Dr. Soetomo Hospital, consisting of 11 males and 40 females, were studied. They were comparing using ELFA and ELISA to know the levels of T4. In this study the method used is a cross sectional observational, and the statistical analysis with nonparametric Spearman Correlation Rank’s test and Wilcoxon Signed Rank’s Test. In the results by using ELFA were found the mean and SD values 8.78 μg/dL /5.39 μg/dL, whereas by ELISA 11.06 μg/dL /5.38 μg/dL. These results showed a correlation between ELFA and ELISA with rs 0.576. The T4 levels results showed as follows, hypothyroid  ELFA 8 (15.7%), ELISA 6 (11.8%) ; normal levels ELFA 32 (62.7%), ELISA 29  (56.9%) ;  hyperthyroid  ELFA 11  (21.6%),  ELISA  16  (31.4%) with p 0.090 which was not considered significant It can be concluded from the showen results that in the determination of T4 concentration using ELFA gave a lower yield compared with ELISA. Further examination is required by special treatment of the sample or other way to find out which one is the best way for determination of T4 levels.     Penentuan kadar tiroksin (T4) diketahui sebagai petunjuk yang baik untuk mengetahui fungsi tiroid. Dalam keadaan hipertirod, kadar T4 jelas meningkat, sedangkan dalam keadaan hipotiroid kadarnya selalu menurun. Perubahan kadar T4 dipengaruhi oleh keadaan fisiologis dan patologis bergantung pada kemampuan globulin yang terikat tiroksin/thyroxin binding globulin (TBG). Penentuan kadar T4 dapat dilakukan dengan enzyme linked immunofluorescent assay (ELFA) dengan enzyme linked immunosorbant assay (ELISA). Pada ELFA dan ELISA yang ditemukan adalah antigen T4.  Dalam kajian ini yang diperiksa kadar T4 dengan ELFA dan ELISA dilakukan di 51 sampel yang diambil secara acak terdiri dari 11 laki-laki dan 40 perempuan di Laboratorium Patologi Klinik RSUD. Dr. Soetomo. Kajian merupakan penelitian cross sectional observation tertentu menggunakan analisis statistik uji non parametrik derajat kenasaban Spearman (Spearman Correlation Rank’s test) dan uji derajat tanda Wilcoxon (Wilcoxon Signed Rank’s Test).  Dalam kajian ini ditemukan nilai mean dan SD ELFA adalah 8.78 μg/dL /5.39 μg/dL, sedangkan ELISA 11.06 μg/dL /5.38 μg/dL. ELFA dan ELISA menunjukkan kenasaban rs 0.576. Kadar T4 hipotiroid  ELFA 8 (15.7%), ELISA 6 (11.8%) ; kadar normal ELFA 32 (62.7%), ELISA 29 (56.9%) ;  hipertiroid  ELFA 11  (21.6%),  ELISA  16  (31.4%) dengan p 0.090 dinyatakan sebagai tidak bermakna Penentuan kadar T4 menggunakan ELFA hasilnya lebih rendah dibandingkan dengan ELISA.  Diperlukan pemeriksan lanjutan  dengan perlakuan khusus terhadap sampel atau cara lain untuk mengetahui cara mana yang terbaik untuk penentuan kadar T4.

Keyword :

ELFA, ELISA, thyroxine,


References :

BioMerieux. Manual Book of Vidas T4,(2004) BioMerieux. Manual Book of Vidas T4 USA : BioMerieux. Manual Book of Vidas T4

Handojo Indro,(2003) Elisa Competitive. Introduction to Basic Imunoasai Surabaya : Airlangga University Press

Jordan WJ,(2010) Enzyme linked immunosorbent assay. Biomethods Medical Handbook USA : Downloaded in August

Demers ML, Spencer AC,(2002) Thyroid Test for the Clinical Biochemistry and Physician USA : Laboratory Medicine Practice Guidelines. NACB

Nelson JC, Wilcox RL,(1999) Analytical Performance of Free and Total Thyroxine Assays USA : Clinical Chemistry





Archive Article

Cover Media Content

Volume : 18 / No. : 1 / Pub. : 2011-01
  1. Aerob Microbes Pattern And Antimicrobial Sensitivity Of Diabetic Foot Ulcer
  2. Plasma Levels Of Interleukin 10 (il-10) In Malaria And Anaemia
  3. Identification Of Cryptosporidiosis In Paediatric Hiv-infected Patients With Chronic Diarrhoea At Paediatric Gastro
  4. Immunoglobulin A In Dengue Hemorrhagic Fever
  5. (comparison Of Determination For Thyroxine With Enzyme Linked Immunofluorescent Assay (elfa) And Enzyme Linked Immunosorbant Assay (elisa)}
  6. Plasma Interleukin-10 And Cd4+ Lymphocyte-t In Hiv Infected Patients
  7. Detecting Fluoroquinolone Resistance Of Salmonella Sp Using Nalidixic Acid Succeptibility Test
  8. Phyllanthus Niruri L The Effects Of Extract On Cellular Immunity Mice
  9. Fitoestrogen Dalam Beberapa Daun Dan Buah
  10. Diagnostic Test Nt Pro Natriuretic Peptide (ntprobnp) On Congestive Heart Failure
  11. Human Immunodeficiency Virus (hiv) Infection In Babies And Children
  12. Decompensated Cirrhosis Hepatic In Children
  13. Human Resources Management In The Clinical Laboratory

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